MTBR-TAU 243/cleaved tau (Val256) Recombinant Rabbit Monoclonal Antibody [PSH20-24]
cat.: HA724135
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: ELISA(Det)
Clonality: Monoclonal
Clone number: PSH20-24
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Synthetic peptide within human MAPT aa 244-256.
Positive control: Recombinant MTBR-TAU 243/cleaved tau (Val256) protein (HA211388).
Subcellular location: Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted.
Recommended Dilutions:
  ELISA(Det)
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-23] to MTBR-TAU 243/cleaved tau (Val256) antibody (Capture) (HA724134) or Rabbit monoclonal [PSH20-25] to MTBR-TAU 243/cleaved tau (Val256) antibody (Capture) (HA724136) and Recombinant MTBR-TAU 243/cleaved tau (Val256) protein (HA211388) as the standard. The reference range value is 5.9-1,500 pg/mL.
Uniprot #: SwissProt: P10636-8 Human
Alternative names: AI413597 AW045860 DDPAC FLJ31424 FTDP 17 G protein beta1/gamma2 subunit interacting factor 1 MAPT MAPTL MGC134287 MGC138549 MGC156663 Microtubule associated protein tau Microtubule associated protein tau isoform 4 Microtubule-associated protein tau MSTD Mtapt MTBT1 MTBT2 Neurofibrillary tangle protein Paired helical filament tau Paired helical filament-tau PHF tau PHF-tau PPND PPP1R103 Protein phosphatase 1, regulatory subunit 103 pTau RNPTAU TAU TAU_HUMAN Tauopathy and respiratory failure, included
Images
HA724135_1.png Fig1: Sandwich ELISA analysis of human eMTBR-Tau 243 matched pair antibodies

Capture: HA724134, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-23]
Detector: HA724135, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-24]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724134) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human eMTBR-Tau 243 protein (HA211388) starting from 1,500 pg/ml to 0 pg/ml and detect antibody (HA724135, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724135_2.png Fig2: Sandwich ELISA analysis of human eMTBR-Tau 243 matched pair antibodies

Capture: HA724134, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-23]
Detector: HA724135, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-24]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724134) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human eMTBR-Tau 243 protein (HA211388) starting from 30,000 pg/ml to 0 pg/ml and detect antibody (HA724135, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724135_3.png Fig3: Sandwich ELISA analysis of human eMTBR-Tau 243 matched pair antibodies

Capture: HA724134, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-23]
Detector: HA724135, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-24]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724134) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human eMTBR-Tau 243 protein (HA211388) starting from 30,000 pg/ml to 0 pg/ml and detect antibody (HA724135, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724135_4.png Fig4: Sandwich ELISA analysis of human eMTBR-Tau 243 matched pair antibodies

Capture: HA724136, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-25]
Detector: HA724135, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-24]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724136) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human eMTBR-Tau 243 protein (HA211388) starting from 1500 pg/ml to 0 pg/ml and detect antibody (HA724135, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724135_5.png Fig5: Sandwich ELISA analysis of human eMTBR-Tau 243 matched pair antibodies

Capture: HA724136, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-25]
Detector: HA724135, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-24]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724136) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human eMTBR-Tau 243 protein (HA211388) starting from 30,000 pg/ml to 0 pg/ml and detect antibody (HA724135, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724135_6.png Fig6: Sandwich ELISA analysis of human eMTBR-Tau 243 matched pair antibodies

Capture: HA724136, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-25]
Detector: HA724135, MTBR-TAU 243/cleaved tau (Val256) Rabbit mAb [PSH20-24]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724136) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human eMTBR-Tau 243 protein (HA211388) starting from 30,000 pg/ml to 0 pg/ml and detect antibody (HA724135, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724135_7.png Fig7: Coating Ab: HA724134, 5ug/ml
Co-Incubation:
1:2 serially diluted human MBTR (aa1-256) and Tau 441 protein from HuaBio starting from a concentration of 30,000 pg/ml and Detect Ab: HA724135, Biotin, 0.2 µg/ml (30℃,120rpm,60min). SA-HRP: 1:20,000 (50ng/ml) (30℃,120rpm,30min).
HA724135_8.png Fig8: Coating Ab: HA724136, 5ug/ml
Co-Incubation:
1:2 serially diluted human MBTR (aa1-256) and Tau 441 protein from HuaBio starting from a concentration of 30,000 pg/ml and Detect Ab: HA724135, Biotin, 0.2 µg/ml (30℃,120rpm,60min). SA-HRP: 1:20,000 (50ng/ml) (30℃,120rpm,30min).
HA724135_9.png
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.