Biotin Conjugated Human FAM171A2 Recombinant Rabbit Monoclonal Antibody [PSH20-27]
cat.: HA724138B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-27 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FAM171A2 aa 20-315 (HA211386). |
| Positive control: | Recombinant Human FAM171A2 protein (HA211386). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-26] to Human FAM171A2 antibody (Capture) (HA724137) or Rabbit monoclonal [PSH20-28] to Human FAM171A2 antibody (Capture) (HA724139) and Recombinant Human FAM171A2 protein (HA211386) as the standard. The reference range value is 39-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: A8MVW0 Human |
| Alternative names: | Protein FAM171A2 FAM171A2 |
Images
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Fig1:
Sandwich ELISA analysis of human FAM171A2 matched pair antibodies Capture: HA724137, Human FAM171A2 Rabbit mAb [PSH20-26] Detector: HA724138, Human FAM171A2 Rabbit mAb [PSH20-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724137) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAM171A2 protein (HA211386) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724138, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Sandwich ELISA analysis of human FAM171A2 matched pair antibodies Capture: HA724139, Human FAM171A2 Rabbit mAb [PSH20-28] Detector: HA724138, Human FAM171A2 Rabbit mAb [PSH20-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724139) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAM171A2 protein (HA211386) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724138, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of spiked FAM171A2 in cell culture media samples. Capture: HA724137, Human FAM171A2 Rabbit mAb [PSH20-26] Detector: HA724138, Human FAM171A2 Rabbit mAb [PSH20-27] The concentrations of FAM171A2 were measured in duplicates, interpolated from the FAM171A2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig4:
Interpolated concentrations of spiked FAM171A2 in cell culture media samples. Capture: HA724139, Human FAM171A2 Rabbit mAb [PSH20-28] Detector: HA724138, Human FAM171A2 Rabbit mAb [PSH20-27] The concentrations of FAM171A2 were measured in duplicates, interpolated from the FAM171A2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.