RUNX1 Recombinant Rabbit Monoclonal Antibody [PSH20-30]
cat.: HA724141
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, ChIP |
| Clonality: | Monoclonal |
| Clone number: | PSH20-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Positive control: | Human breast cancer tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P ChIP |
1:1,000 Use 0.5~2 μg for 25 μg of chromatin. |
| Uniprot #: | SwissProt: Q01196 Human |
| Alternative names: | Runt-related transcription factor 1 Acute myeloid leukemia 1 protein Core-binding factor subunit alpha-2 (CBF-alpha-2) Oncogene AML-1 Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B; PEBP2-alpha B) SL3-3 enhancer factor 1 alpha B subunit SL3/AKV core-binding factor alpha B subunit RUNX1 AML1, CBFA2 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-RUNX1 antibody (HA724141) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724141) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells with RUNX1 (HA724141) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.