NCX1 Recombinant Rabbit Monoclonal Antibody [PSH20-37]
cat.: HA724148
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH20-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NCX1 aa 253-800. |
| Positive control: | Mouse heart tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, human brain tissue, human heart tissue, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IF-Tissue |
1:5,000 1:500 1:200-1:1,000 1:200 |
| Uniprot #: | SwissProt: P32418 Human | P70414 Mouse | Q01728 Rat |
| Alternative names: | CNC DKFZp779F0871 MGC119581 Na(+)/Ca(2+)-exchange protein 1 Na+/Ca2+ exchange protein 1 Na+/Ca2+ exchanger NAC1_HUMAN NCX 1 NCX NCX1 SLC8A1 SLC8A1 protein Sodium Calcium Exchanger Sodium/calcium exchanger 1 Solute carrier family 8 (sodium/calcium exchanger) member 1 Solute carrier family 8 member 1 |
Images
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Fig1:
Western blot analysis of NCX1 on different lysates with Rabbit anti-NCX1 antibody (HA724148) at 1/5,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse liver tissue lysate (low expression) Lane 4: Rat heart tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 109 kDa Observed band size: 109 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724148) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: heart Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Rat Site: heart Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NCX1 antibody (HA724148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-NCX1 antibody (HA724148) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724148) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human liver tissue (hepatocytes negative) with Rabbit anti-NCX1 antibody (HA724148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NCX1 antibody (HA724148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-NCX1 antibody (HA724148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-NCX1 antibody (HA724148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-NCX1 antibody (HA724148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.