TRPV1 Recombinant Rabbit Monoclonal Antibody [PSH20-38]
cat.: HA724149
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH20-38 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRPV1 aa 94-433. |
| Positive control: | Mouse dorsal root ganglion tissue, rat dorsal root ganglion tissue. |
| Subcellular location: | Postsynaptic cell membrane, Cell projection, dendritic spine membrane, Cell membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P IF-Tissue |
1:1,000-1:5,000 1:15,000 1:10,000-1:15,000 |
| Uniprot #: | SwissProt: Q704Y3 Mouse | O35433 Rat |
| Alternative names: | Capsaicin receptor DKFZp434K0220 osm 9 like TRP channel 1 Osm-9-like TRP channel 1 OTRPC1 Transient receptor potential cation channel subfamily V member 1 TRPV 1 Trpv1 TRPV1_HUMAN Vanilloid receptor 1 Vanilloid receptor subtype 1 VR 1 VR1 |
Images
|
Fig1:
Application: IHC-Fr Species: Mouse Site: dorsal root ganglion Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required |
|
Fig2:
Application: IHC-Fr Species: Rat Site: dorsal root ganglion Sample: Frozen section Antibody concentration: 1/5,000 Antigen retrieval: Not required |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse dorsal root ganglion tissue with Rabbit anti-TRPV1 antibody (HA724149) at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724149) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue with Rabbit anti-TRPV1 antibody (HA724149) at 1/15,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724149) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-TRPV1 antibody (HA724149) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724149) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Application: IF-Tissue Species: Mouse Site: dorsal root ganglion Sample: Paraffin-embedded section Antibody concentration: 1/10,000 |
|
Fig7:
Application: IF-Tissue Species: Rat Site: dorsal root ganglion Sample: Paraffin-embedded section Antibody concentration: 1/15,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.