CHX10 Recombinant Rabbit Monoclonal Antibody [PSH20-42]
cat.: HA724153
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH20-42 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CHX10 aa 1-361. |
| Positive control: | Mouse eyeball tissue, rat eyeball tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:200-1:500 1:1,000-1:4,000 |
| Uniprot #: | SwissProt: Q61412 Mouse Entrez Gene: 171360 Rat |
| Alternative names: | C elegans ceh 10 homeo domain containing homolog Ceh 10 homeo domain containing homolog (C. elegans) Ceh 10 homeo domain containing homolog Ceh 10 homeodomain containing homolog Ceh 10 homeodomain containing homolog (C. elegans) Ceh-10 homeodomain-containing homolog Ceh10 homeo domain containing homolog Ceh10 homeodomain containing homolog CHX 10 Homeobox protein CHX 10 Homeobox protein CHX10 HOX 10 HOX10 MCOP 2 MCOP2 MCOPCB 3 MCOPCB3 RET 1 RET1 Visual system homeobox 2 Vsx 2 Vsx2 VSX2_HUMAN |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: eyeball Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Application: IHC-Fr Species: Rat Site: eyeball Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-CHX10 antibody (HA724153) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724153) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (negative) with Rabbit anti-CHX10 antibody (HA724153) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724153) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-CHX10 antibody (HA724153) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724153) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.