IGFBP6 Recombinant Rabbit Monoclonal Antibody [PSH20-44]
cat.: HA724155
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH20-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IGFBP6 aa 1-240. |
| Positive control: | U-87 MG cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, NCI-H1299 cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P24592 Human |
| Alternative names: | IBP 6 IBP-6 IBP6 IBP6_HUMAN IGF binding protein 6 IGF-binding protein 6 IGFBP 6 IGFBP-6 IGFBP6 Insulin like growth factor binding protein 6 Insulin-like growth factor-binding protein 6 |
Images
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Fig1:
Western blot analysis of IGFBP6 on different lysates with Rabbit anti-IGFBP6 antibody (HA724155) at 1/5,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: HeLa cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: NCI-H1299 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 35 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724155) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.