Biotin Conjugated Rat CXCL2 Recombinant Rabbit Monoclonal Antibody [PSH20-56]
cat.: HA724158B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat CXCL2 aa 32-100 (HA211132). |
| Positive control: | Recombinant Rat CXCL2 protein (HA211132). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-55] to Rat CXCL2 antibody (Capture) (HA724157) and Recombinant Rat CXCL2 protein (HA211132) as the standard. The reference range value is 11.7-3,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P30348 Rat |
| Alternative names: | C-X-C motif chemokine 2 Chemokine (C X C motif) ligand 2 Chemokine, CXC motif, ligand 2 CINC 2a CINC2a CINC3 CXC chemokine CXCL 2 Cxcl2 Cytokine-induced neutrophil chemoattractant 3 GRO 2 GRO b GRO protein, beta Gro-beta GRO-beta(5-73) GRO-beta-T GRO2 GRO2 oncogene GROb GRObeta Growth regulated protein beta Growth-regulated protein beta GROX Hematopoietic synergistic factor HSF Macrophage inflammatory protein 2 alpha Macrophage inflammatory protein 2 Macrophage inflammatory protein 2-alpha Melanoma growth stimulatory activity beta MGSA b MGSA beta MIP 2 MIP 2a MIP2 alpha MIP2 MIP2-alpha MIP2A MIP2alpha SB-251353 SCYB 2 Scyb SCYB2 Small inducible cytokine subfamily B, member 2 |
Images
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Fig1:
Sandwich ELISA analysis of Rat CXCL2 matched pair antibodies Capture: HA724157, Rat CXCL2 Rabbit mAb [PSH20-55] Detector: HA724158, Rat CXCL2 Rabbit mAb [PSH20-56] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724157) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat CXCL2 protein (HA211132) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA724158, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CXCL2 in Rat heart supernatant treated with Con A for 72h. Capture: HA724157, Rat CXCL2 Rabbit mAb [PSH20-55] Detector: HA724158, Rat CXCL2 Rabbit mAb [PSH20-56] Interpolated concentration of native CXCL2 was measured in duplicate at different sample concentrations and interpolated from the CXCL2 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL2 concentration was determined to be 14,021 pg/ml in Rat heart treated supernatant. |
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Fig3:
Interpolated concentrations of native CXCL2 in Rat lung supernatant treated with Con A for 72h. Capture: HA724157, Rat CXCL2 Rabbit mAb [PSH20-55] Detector: HA724158, Rat CXCL2 Rabbit mAb [PSH20-56] Interpolated concentration of native CXCL2 was measured in duplicate at different sample concentrations and interpolated from the CXCL2 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL2 concentration was determined to be 113,735 pg/ml in Rat lung treated supernatant. |
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Fig4:
Interpolated concentrations of spiked CXCL2 in cell culture media samples. Capture: HA724157, Rat CXCL2 Rabbit mAb [PSH20-55] Detector: HA724158, Rat CXCL2 Rabbit mAb [PSH20-56] The concentrations of CXCL2 were measured in duplicates, interpolated from the CXCL2 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.