Biotin Conjugated Rat TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH20-58]
cat.: HA724160B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-58 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat TIMP1 aa 24-217 (HA211163). |
| Positive control: | Recombinant Rat TIMP1 protein (HA211163). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-57] to Rat TIMP1 antibody (Capture) (HA724159) and Recombinant Rat TIMP1 protein (HA211163) as the standard. The reference range value is 7.8-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P30120 Rat |
| Alternative names: | Clgi Collagenase inhibitor EPA EPO Erythroid Potentiating Activity Erythroid-potentiating activity Fibroblast collagenase inhibitor FLJ90373 HCI Human Collagenase Inhibitor Metalloproteinase inhibitor 1 Metalloproteinase inhibitor 1 precursor OTTHUMP00000023214 TIMP 1 TIMP TIMP metallopeptidase inhibitor 1 TIMP-1 Timp1 TIMP1 protein Tissue Inhibitor of Metalloproteinase 1 Tissue inhibitor of metalloproteinases 1 Tissue inhibitor of metalloproteinases Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor |
Images
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Fig1:
Sandwich ELISA analysis of Rat TIMP1 matched pair antibodies Capture: HA724159, Rat TIMP1 Rabbit mAb [PSH20-57] Detector: HA724160, Rat TIMP1 Rabbit mAb [PSH20-58] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724159) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat TIMP1 protein (HA211163) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA724160, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native TIMP1 in Rat serum. Capture: HA724159, Rat TIMP1 Rabbit mAb [PSH20-57] Detector: HA724160, Rat TIMP1 Rabbit mAb [PSH20-58] Interpolated concentration of native TIMP1 was measured in duplicate at different sample concentrations and interpolated from the TIMP1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 6,137 pg/ml in Rat serum. |
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Fig3:
Interpolated concentrations of native TIMP1 in Rat spleen supernatant for 72h. Capture: HA724159, Rat TIMP1 Rabbit mAb [PSH20-57] Detector: HA724160, Rat TIMP1 Rabbit mAb [PSH20-58] Interpolated concentration of native TIMP1 was measured in duplicate at different sample concentrations and interpolated from the TIMP1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 523 pg/ml in Rat spleen supernatant. |
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Fig4:
Interpolated concentrations of native TIMP1 in Rat lung supernatant for 72h. Capture: HA724159, Rat TIMP1 Rabbit mAb [PSH20-57] Detector: HA724160, Rat TIMP1 Rabbit mAb [PSH20-58] Interpolated concentration of native TIMP1 was measured in duplicate at different sample concentrations and interpolated from the TIMP1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 870 pg/ml in Rat lung supernatant. |
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Fig5:
Interpolated concentrations of spiked TIMP1 in cell culture media samples. Capture: HA724159, Rat TIMP1 Rabbit mAb [PSH20-57] Detector: HA724160, Rat TIMP1 Rabbit mAb [PSH20-58] The concentrations of TIMP1 were measured in duplicates, interpolated from the TIMP1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.