Human Amphiregulin Recombinant Rabbit Monoclonal Antibody [PSH20-59]
cat.: HA724161
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH20-59 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Amphiregulin aa 20-187 (HA211382). |
| Positive control: | Recombinant Human Amphiregulin protein (HA211382). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-60] to Human Amphiregulin antibody (Detector) (HA724162) and Recombinant Human Amphiregulin protein (HA211382) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: P15514 Human |
| Alternative names: | A REG Amphiregulin Amphiregulin B AR AREG AREG_HUMAN AREGB Colorectum cell derived growth factor Colorectum cell-derived growth factor CRDGF MGC13647 Schwannoma derived growth factor SDGF |
Images
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Fig1:
Sandwich ELISA analysis of human Amphiregulin matched pair antibodies Capture: HA724161, Human Amphiregulin Rabbit mAb [PSH20-59] Detector: HA724162, Human Amphiregulin Rabbit mAb [PSH20-60] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724161) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Amphiregulin protein (HA211382) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA724162, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Amphiregulin in MCF7 supernatant treated or untreated with PMA for 48 hours. Capture: HA724161, Human Amphiregulin Rabbit mAb [PSH20-59] Detector: HA724162, Human Amphiregulin Rabbit mAb [PSH20-60] Interpolated concentration of native Amphiregulin was measured in duplicate at different sample concentrations and interpolated from the Amphiregulin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Amphiregulin concentration was determined to be 7,785 pg/mL in neat MCF7 treated supernatant and 1,048 pg/ml in untreated MCF7 supernatant. |
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Fig3:
Interpolated concentrations of spiked Amphiregulin in cell culture media samples. Capture: HA724161, Human Amphiregulin Rabbit mAb [PSH20-59] Detector: HA724162, Human Amphiregulin Rabbit mAb [PSH20-60] The concentrations of Amphiregulin were measured in duplicates, interpolated from the Amphiregulin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.