DUX4 Recombinant Rabbit Monoclonal Antibody [PSH20-66]
cat.: HA724165
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: PSH20-66
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Positive control: HeLa cell lysate, HepG2 cell lysate, Mouse testis tissue lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9UBX2 Human
Alternative names: Double homeobox 4 Double homeobox protein 10 Double homeobox protein 4 Double homeobox protein 4/10 DUX10
Images
HA724165_1.jpg Fig1: Western blot analysis of DUX4 on different lysates with Rabbit anti-DUX4 antibody (HA724165) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (40 µg/Lane)

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724165) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.