Lipocalin-2 Recombinant Rabbit Monoclonal Antibody [PSH20-68]
cat.: HA724166
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH20-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Lipocalin-2 aa 1-198. |
| Positive control: | HaCaT cell lysate, HT-29 cell lysate, HT-29. |
| Subcellular location: | Secreted, Cytoplasmic granule lumen, Cytoplasmic vesicle lumen. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P80188 Human |
| Alternative names: | 24p3 25 kDa alpha 2 microglobulin related subunit of MMP9 25 kDa alpha-2-microglobulin-related subunit of MMP-9 Alpha 2 microglobulin related protein HGNC:6526 HNL Lcn 2 Lcn2 Lipocalin-2 Migration stimulating factor inhibitor MSFI Neutrophil gelatinase associated lipocalin Neutrophil gelatinase associated lipocalin precursor Neutrophil gelatinase-associated lipocalin NGAL NGAL_HUMAN Oncogene 24p3 Oncogenic lipocalin 24p3 p25 Siderocalin siderocalin LCN2 SV40 induced 24P3 protein Uterocalin |
Images
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Fig1:
Western blot analysis of Lipocalin-2 on different lysates with Rabbit anti-Lipocalin-2 antibody (HA724166) at 1/5,000 dilution. Lane 1: HaCaT cell lysate Lane 2: HT-29 cell lysate Lane 3: SH-SY5Y cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724166) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HT-29 cells labeling Lipocalin-2 with Rabbit anti-Lipocalin-2 antibody (HA724166) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lipocalin-2 antibody (HA724166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HT-29 cells labeling Lipocalin-2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA724166, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.