Biotin Conjugated Human ICAM3 Recombinant Rabbit Monoclonal Antibody [PSH20-70]
cat.: HA724168B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ICAM3 aa 30-485 (HA211145). |
| Positive control: | Recombinant Human ICAM3 protein (HA211145). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-69] to Human ICAM3 antibody (Capture) (HA724167) and Recombinant Human ICAM3 protein (HA211145) as the standard. The reference range value is 78.1-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P32942 Human |
| Alternative names: | CD50 CDW 50 CDw50 ICAM 3 ICAM-3 ICAM-R ICAM3 ICAM3_HUMAN ICAMR Intercellular adhesion molecule 3 |
Images
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Fig1:
Sandwich ELISA analysis of Human ICAM3 matched pair antibodies Capture: HA724167, Human ICAM3 Rabbit mAb [PSH20-69] Detector: HA724168, Human ICAM3 Rabbit mAb [PSH20-70] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724167) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ICAM3 protein (HA211145) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724168, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native ICAM-3 in human serum samples. Capture: HA724167, Human ICAM3 Rabbit mAb [PSH20-69] Detector: HA724168, Human ICAM3 Rabbit mAb [PSH20-70] The concentrations of ICAM-3 were measured in duplicates, interpolated from the ICAM-3 standard curve and corrected for sample dilution. Undiluted samples are human serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM-3 concentration was determined to be 216 ng/ml in human serum. |
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Fig3:
Interpolated concentrations of spiked ICAM-3 in human cell culture media samples. Capture: HA724167, Human ICAM3 Rabbit mAb [PSH20-69] Detector: HA724168, Human ICAM3 Rabbit mAb [PSH20-70] The concentrations of ICAM-3 were measured in duplicates, interpolated from the ICAM-3 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.