IRAK4 Recombinant Rabbit Monoclonal Antibody [PSH20-81]
cat.: HA724176
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH20-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Positive control: | Jurkat cell lysate, THP-1 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, MEF cell lysate, J774A.1 cell lysate, THP-1. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q9NWZ3 Human | Q8R4K2 Mouse |
| Alternative names: | IL-1 receptor-associated kinase 4 Interleukin 1 receptor associated kinase 4 mutant form 1 Interleukin-1 receptor-associated kinase 4 Interleukin1 receptor associated kinase 4 IPD1 IRAK 4 IRAK-4 IRAK4 IRAK4 mutated form 1 IRAK4_HUMAN LOC 51135 NY REN 64 NY REN 64 antigen NY-REN-64 REN64 Renal carcinoma antigen NY-REN-64 |
Images
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Fig1:
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (HA724176) at 1/5,000 dilution. Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: MCF7 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: MEF cell lysate Lane 6: J774A.1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724176) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of THP-1 cells labeling IRAK4 with Rabbit anti-IRAK4 antibody (HA724176) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRAK4 antibody (HA724176) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of THP-1 cells labeling IRAK4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA724176, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.