Biotin Conjugated Mouse TSLP Recombinant Rabbit Monoclonal Antibody [PSH20-84]
cat.: HA724179B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-84 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Mouse TSLP aa 20-470 (HA211088). |
| Positive control: | Recombinant Mouse TSLP protein (HA211088). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-83] to Mouse TSLP antibody (Capture) (HA724178) and Recombinant Mouse TSLP protein (HA211088) as the standard. The reference range value is 7.8-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9JIE6 Mouse |
| Alternative names: | Thymic stromal lymphopoietin Thymic stromal lymphopoietin protein TSLP Tslp TSLP protein |
Images
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Fig1:
Sandwich ELISA analysis of Mouse TSLP matched pair antibodies Capture: HA724178, Mouse TSLP Rabbit mAb [PSH20-83] Detector: HA724179, Mouse TSLP Rabbit mAb [PSH20-84] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724178) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse TSLP protein (HA211088) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA724179, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native TSLP in mouse heart and mouse lung cultured for 5 days culture supernatant. Capture: HA724178, Mouse TSLP Rabbit mAb [PSH20-83] Detector: HA724179, Mouse TSLP Rabbit mAb [PSH20-84] The concentrations of TSLP were measured in duplicates, interpolated from the TSLP standard curve and corrected for sample dilution. Undiluted samples are mouse heart culture supernatant 100% and mouse lung culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TSLP concentration was determined to be 206.3 pg/ml in mouse heart culture supernatant and 140.5 pg/ml in mouse lung culture supernatant. |
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Fig3:
Interpolated concentrations of spiked TSLP in mouse cell culture media samples. Capture: HA724178, Mouse TSLP Rabbit mAb [PSH20-83] Detector: HA724179, Mouse TSLP Rabbit mAb [PSH20-84] The concentrations of TSLP were measured in duplicates, interpolated from the TSLP standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.