Biotin Conjugated Human DLL1 Recombinant Rabbit Monoclonal Antibody [PSH20-86]
cat.: HA724181B
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: ELISA(Det), ELISA
Clonality: Monoclonal
Clone number: PSH20-86
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Recombinant Human DLL1 aa 24-188 (HA210644).
Positive control: Recombinant Human DLL1 protein (HA210644).
Subcellular location: Apical cell membrane, Cell junction, adherens junction, Membrane raft.
Recommended Dilutions:
  ELISA(Det)
  ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-85] to Human DLL1 antibody (Capture) (HA724180) or Rabbit monoclonal [PSH20-87] to Human DLL1 antibody (Capture) (HA724182) and Recombinant Human DLL1 protein (HA210644) as the standard. The reference range value is 39.1-10,000 pg/mL.
Use at an assay dependent concentration.
Uniprot #: SwissProt: O00548 Human
Alternative names: Delta (Drosophila) like 1 Delta Delta like 1 (Drosophila) Delta like 1 Delta like 1 protein Delta like protein 1 Delta like protein 1 precursor Delta Drosophila homolog of 1 Delta-like protein 1 Delta1 DL1 DLL 1 DLL1 DLL1_HUMAN Drosophila Delta homolog 1 H-Delta-1 OTTHUMP00000017690
Images
HA724181B_1.png Fig1: Sandwich ELISA analysis of Human DLL1 matched pair antibodies

Capture: HA724180, Human DLL1 Rabbit mAb [PSH20-85]
Detector: HA724181, Human DLL1 Rabbit mAb [PSH20-86]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724180) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted DLL1 protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724181, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724181B_2.png Fig2: Sandwich ELISA analysis of Human DLL1 matched pair antibodies

Capture: HA724182, Human DLL1 Rabbit mAb [PSH20-87]
Detector: HA724181, Human DLL1 Rabbit mAb [PSH20-86]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724180) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted DLL1 protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724182, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724181B_3.png Fig3: Interpolated concentrations of native DLL1 in Human serum.

Capture: HA724180, Human DLL1 Rabbit mAb [PSH20-85]
Detector: HA724181, Human DLL1 Rabbit mAb [PSH20-86]

Interpolated concentration of native DLL1 was measured in duplicate at different sample concentrations and interpolated from the DLL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean DLL1 concentration was determined to be 11,292 pg/ml in Human serum.
HA724181B_4.png Fig4: Interpolated concentrations of native DLL1 in Human serum.

Capture: HA724182, Human DLL1 Rabbit mAb [PSH20-87]
Detector: HA724181, Human DLL1 Rabbit mAb [PSH20-86]

Interpolated concentration of native DLL1 was measured in duplicate at different sample concentrations and interpolated from the DLL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean DLL1 concentration was determined to be 7,022 pg/ml in Human serum.
HA724181B_5.png Fig5: Interpolated concentrations of native DLL1 in AGS and MCF-7 cell culture supernatant.

Capture: HA724180, Human DLL1 Rabbit mAb [PSH20-85]
Detector: HA724181, Human DLL1 Rabbit mAb [PSH20-86]

Interpolated concentration of native DLL1 was measured in duplicate at different sample concentrations and interpolated from the DLL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean DLL1 concentration was determined to be 436 pg/ml in MCF-7 cell culture supernatant and undetectable in AGS cell culture supernatant.
HA724181B_6.png Fig6: Interpolated concentrations of native DLL1 in AGS and MCF-7 cell culture supernatant.

Capture: HA724182, Human DLL1 Rabbit mAb [PSH20-87]
Detector: HA724181, Human DLL1 Rabbit mAb [PSH20-86]

Interpolated concentration of native DLL1 was measured in duplicate at different sample concentrations and interpolated from the DLL1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean DLL1 concentration was determined to be 532 pg/ml in MCF-7 cell culture supernatant and undetectable in AGS cell culture supernatant.
HA724181B_7.png Fig7: Interpolated concentrations of spiked DLL1 in cell culture media samples.

Capture: HA724180, Human DLL1 Rabbit mAb [PSH20-85]
Detector: HA724181, Human DLL1 Rabbit mAb [PSH20-86]

The concentrations of DLL1 were measured in duplicates, interpolated from the DLL1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA724181B_8.png Fig8: Interpolated concentrations of spiked DLL1 in cell culture media samples.

Capture: HA724182, Human DLL1 Rabbit mAb [PSH20-87]
Detector: HA724181, Human DLL1 Rabbit mAb [PSH20-86]

The concentrations of DLL1 were measured in duplicates, interpolated from the DLL1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.