Mouse Periostin Recombinant Rabbit Monoclonal Antibody [PSH20-89]
cat.: HA724184
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH20-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Mouse Periostin aa 24-838 (HA211398). |
| Positive control: | Recombinant Mouse Periostin protein (HA211398). |
| Subcellular location: | Golgi apparatus, Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-88] to Mouse Periostin antibody (Capture) (HA724183) and Recombinant Mouse Periostin protein (HA211398) as the standard. The reference range value is 78.1-20,000 pg/mL. |
| Uniprot #: | SwissProt: Q62009 Mouse |
| Alternative names: | Fasciclin-I like MGC119510 MGC119511 OSF 2 OSF-2 OSF2 Osteoblast specific factor 2 (fasciclin I like) Osteoblast specific factor 2 Osteoblast specific factor Osteoblast-specific factor 2 PDLPOSTN Periodontal ligament specific periostin Periostin Periostin isoform thy2 Periostin isoform thy4 Periostin isoform thy6 Periostin isoform thy8 Periostin osteoblast specific factor PN POSTN RP11 412K4.1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Periostin matched pair antibodies Capture: HA724183, Mouse Periostin Rabbit mAb [PSH20-88] Detector: HA724184, Mouse Periostin Rabbit mAb [PSH20-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724183) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Periostin protein (HA211398) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA724184, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Periostin in mouse serum and Neuro-2a cell culture supernatant samples. Capture: HA724183, Mouse Periostin Rabbit mAb [PSH20-88] Detector: HA724184, Mouse Periostin Rabbit mAb [PSH20-89] The concentrations of Periostin were measured in duplicates, interpolated from the Periostin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.1% and Neuro-2a cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Periostin concentration was determined to be 6,579 ng/ml in mouse serum and undetectabel in Neuro-2a cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked Periostin in mouse cell culture media samples. Capture: HA724183, Mouse Periostin Rabbit mAb [PSH20-88] Detector: HA724184, Mouse Periostin Rabbit mAb [PSH20-89] The concentrations of Periostin were measured in duplicates, interpolated from the Periostin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.