Biotin Conjugated Mouse/Rat IGFBP2 Recombinant Rabbit Monoclonal Antibody [PSH21-22]
cat.: HA724205B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Mouse IGFBP2 aa 35-305 (HA211197). |
| Positive control: | Recombinant Mouse IGFBP2 protein (HA211197). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-21] to Mouse/Rat IGFBP2 antibody (Capture) (HA724204) or Rabbit monoclonal [PSH21-23] to Mouse/Rat IGFBP2 antibody (Capture) (HA724206) and Recombinant Mouse IGFBP2 protein (HA211197) as the standard. The reference range value is 62.5-8,000 pg/mL. |
| Uniprot #: | SwissProt: P47877 Mouse | P12843 Rat |
| Alternative names: | BP 2 BP2 IBP 2 IBP-2 IBP2 IBP2_HUMAN IGF binding protein 2 IGF BP53 IGF-binding protein 2 IGFBP 2 IGFBP-2 IGFBP2 IGFBP53 Insulin like growth factor binding protein 2 36kDa Insulin like growth factor binding protein 2 Insulin like growth factor-binding protein 2 precursor Insulin-like growth factor-binding protein 2 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IGFBP2 matched pair antibodies Capture: HA724204, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-21] Detector: HA724205, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724204) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IGFBP2 protein (HA211197) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA724205, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Sandwich ELISA analysis of Mouse IGFBP2 matched pair antibodies Capture: HA724206, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-23] Detector: HA724205, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724206) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IGFBP2 protein (HA211197) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA724205, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig3:
Interpolated concentrations of native IGFBP2 in mouse and rat samples. Capture: HA724204, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-21] Detector: HA724205, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-22] Interpolated concentration of native IGFBP2 was measured in duplicate at different sample concentrations and interpolated from the IGFBP2 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 459 ng/mL in mouse serum and 41.9 ng/mL in rat serum. |
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Fig4:
Interpolated concentrations of native IGFBP2 in mouse and rat samples. Capture: HA724206, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-23] Detector: HA724205, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-22] Interpolated concentration of native IGFBP2 was measured in duplicate at different sample concentrations and interpolated from the IGFBP2 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 517 ng/mL in mouse serum and 71.2 ng/mL in rat serum. |
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Fig5:
Interpolated concentrations of spiked IGFBP2 in cell culture media samples. Capture: HA724204, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-21] Detector: HA724205, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-22] The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
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Fig6:
Interpolated concentrations of spiked IGFBP2 in cell culture media samples. Capture: HA724206, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-23] Detector: HA724205, Mouse/Rat IGFBP2 Rabbit mAb [PSH21-22] The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.