NCR1 Recombinant Rabbit Monoclonal Antibody [PSH21-26]
cat.: HA724209
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH21-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse NCR1 aa 86-255. |
| Positive control: | Mouse colon tissue, mouse spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-P FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q8C567 Mouse |
| Alternative names: | Activating NK receptor NK p46 CD335 FLJ99094 hNKp46 Ly94 Lymphocyte antigen 94 Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) Lymphocyte antigen 94 homolog Natural cytotoxicity triggering receptor 1 Natural killer cell p46-related protein NCR1 NCT1 NCTR1_HUMAN NK cell activating receptor NK cell-activating receptor NK-p46 NKp46 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-NCR1 antibody (HA724209) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-NCR1 antibody (HA724209) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724209) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Flow cytometric analysis of C57BL/6 mouse spleen cells labeling NCR1 (HA724209) and NK1.1 (HA720219F, FITC). Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA724209, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 647 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1123) at 1/1,000 dilution for 30 minutes at +4℃. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.