CXCR2 Recombinant Rabbit Monoclonal Antibody [PSH21-28]
cat.: HA724211
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH21-28 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.05ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CXCR2 aa 316-360. |
| Positive control: | A549 cell lysate, MDA-MB-231 cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, Neuro-2a cell lysate, 4T1 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:10,000 |
| Uniprot #: | SwissProt: P25025 Human | P35343 Mouse | P35407 Rat |
| Alternative names: | C-X-C chemokine receptor type 2 CD 182 CD182 CD182 antigen CDw128b Chemokine (CXC) receptor 2 CMKAR2 CXC-R2 CXCR 2 CXCR-2 CXCR2 CXCR2_HUMAN GRO/MGSA receptor High affinity interleukin-8 receptor B IL 8 receptor type 2 IL 8R B IL-8 receptor type 2 IL-8R B IL8 RB IL8 receptor type 2 IL8R B IL8R2 IL8RA Interleukin 8 Receptor B Interleukin 8 receptor, beta Interleukin 8 receptor, type 2 |
Images
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Fig1:
Western blot analysis of CXCR2 on different lysates with Rabbit anti-CXCR2 antibody (HA724211) at 1/10,000 dilution. Lane 1: A549 cell lysate (no heat) (10 µg/Lane) Lane 2: MDA-MB-231 cell lysate (no heat) (10 µg/Lane) Lane 3: SK-Br-3 cell lysate (10 µg/Lane) Lane 4: HepG2 cell lysate (10 µg/Lane) Lane 5: Neuro-2a cell lysate (no heat) (10 µg/Lane) Lane 6: 4T1 cell lysate (10 µg/Lane) Lane 7: PC-12 cell lysate (10 µg/Lane) Lane 8: C6 cell lysate (10 µg/Lane) Lane 9: Mouse spleen tissue lysate (no heat) (20 µg/Lane) Lane 10: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 11: Rat spleen tissue lysate (no heat) (20 µg/Lane) Lane 12: Rat brain tissue lysate (no heat) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 41 kDa Observed band size: 45 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724211) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.