Bassoon / BSN Recombinant Rabbit Monoclonal Antibody [PSH21-32]
cat.: HA724215
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH21-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 419 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse BSN aa 1,201-1,600. |
| Positive control: | Mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, Presynaptic active zone, cytoskeleton, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:500 1:200 |
| Uniprot #: | SwissProt: O88737 Mouse | O88778 Rat |
| Alternative names: | Protein bassoon Bsn Kiaa0434 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Bassoon / BSN antibody (HA724215) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724215) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Bassoon / BSN antibody (HA724215) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724215) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue (negative) with Rabbit anti-Bassoon / BSN antibody (HA724215) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724215) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Bassoon / BSN antibody (HA724215) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724215) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded rat heart tissue (negative) with Rabbit anti-Bassoon / BSN antibody (HA724215) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724215) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.