SAA1 + SAA2 Recombinant Rabbit Monoclonal Antibody [PSH21-34]
cat.: HA724217
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH21-34 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse SAA1 aa 1-122. |
| Positive control: | Mouse plasma tissue lysate, Mouse serum tissue lysate, mouse liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200 |
| Uniprot #: | SwissProt: P05366 Mouse | P05367 Mouse |
| Alternative names: | amyloid A, serum Amyloid fibril protein AA Amyloid protein A MGC111216 PIG4 SAA Serum amyloid A protein serum amyloid A-1 protein serum amyloid A1 TP53I4 Tumor protein p53 inducible protein 4 Serum amyloid A-2 protein Amyloid protein A Saa2 |
Images
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Fig1:
Western blot analysis of SAA1 + SAA2 on different lysates with Rabbit anti-SAA1 + SAA2 antibody (HA724217) at 1/5,000 dilution. Lane 1: Mouse plasma tissue lysate Lane 2: Mouse serum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724217) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SAA1 + SAA2 on different lysates with Rabbit anti-SAA1 + SAA2 antibody (HA724217) at 1/5,000 dilution. Lane 1: no-tagged Mouse SAA1 recombinant protein Lane 2: Avi-Ltag-His-tagged Mouse SAA2 recombinant protein Lane 2: Flag-tagged Human SAA1 recombinant protein Lysates/proteins at 20 ng/Lane. Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724217) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SAA1 + SAA2 antibody (HA724217) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724217) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.