Mouse IL1RL1 / ST2 Recombinant Rabbit Monoclonal Antibody [PSH21-58]
cat.: HA724225
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-58 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Mouse IL1RL1 / ST2 aa 27-332 (HA211193). |
| Positive control: | Recombinant Mouse IL1RL1 / ST2 protein (HA211193). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-59] to Mouse IL1RL1 / ST2 antibody (Detector) (HA724226) and Recombinant Mouse IL1RL1 / ST2 protein (HA211193) as the standard. The reference range value is 78-10,000 pg/mL. |
| Uniprot #: | SwissProt: P14719 Mouse |
| Alternative names: | DER-4 DER4 FIT 1 Growth stimulation expressed homolog of mouse growth stimulation-expressed Il1rl1 IL33R Interleukin 1 receptor like 1 interleukin 1 receptor related protein Interleukin-1 receptor-like 1 Protein ST2 ST2 ST2L ST2V T1 T1 protein |
Images
|
Fig1:
Sandwich ELISA analysis of mouse ST2 matched pair antibodies Capture: HA724225, Mouse IL1RL1 / ST2 Rabbit mAb [PSH21-58] Detector: HA724226, Mouse IL1RL1 / ST2 Rabbit mAb [PSH21-59] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724225) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL1RL1 / ST2 protein (HA211193) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA724226, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native ST2 in mouse samples. Capture: HA724225, Mouse IL1RL1 / ST2 Rabbit mAb [PSH21-58] Detector: HA724226, Mouse IL1RL1 / ST2 Rabbit mAb [PSH21-59] Interpolated concentration of native ST2 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 136,781 pg/mL in NIH-3T3 cell supernatant and 15,551 pg/ml in mouse serum. |
|
Fig3:
Interpolated concentrations of spiked ST2 in cell culture media samples. Capture: HA724225, Mouse IL1RL1 / ST2 Rabbit mAb [PSH21-58] Detector: HA724226, Mouse IL1RL1 / ST2 Rabbit mAb [PSH21-59] The concentrations of ST2 were measured in duplicates, interpolated from the ST2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.