Biotin Conjugated Human Interferon alpha 2 Recombinant Rabbit Monoclonal Antibody [PSH21-61]
cat.: HA724228B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH21-61 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human Interferon alpha 2 aa 24-188 (HA210644). |
| Positive control: | Recombinant Human Interferon alpha 2 protein (HA210644). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-60] to Human Interferon alpha 2 antibody (Capture) (HA724227) and Recombinant Human Interferon alpha 2 protein (HA210644) as the standard. The reference range value is 39-5,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01563 Human |
| Alternative names: | Alpha 2a interferon IFN alpha 2 IFN alphaA IFN IFN-alpha-2 IFNA Ifna2 IFNA2_HUMAN IFNA2B Interferon alpha 2a Interferon alpha 2b Interferon alpha A Interferon alpha-2 Interferon alpha-A Interferon LeIF A LeIFA MGC125764 MGC125765 |
Images
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Fig1:
Sandwich ELISA analysis of Human Interferon alpha 2 matched pair antibodies Capture: HA724227, Human Interferon alpha 2 Rabbit mAb [PSH21-60] Detector: HA724228, Human Interferon alpha 2 Rabbit mAb [PSH21-61] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724227) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Interferon alpha 2 protein (HA210644) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724228, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of spiked IFN alpha-2 in cell culture media samples. Capture: HA724227, Human Interferon alpha 2 Rabbit mAb [PSH21-60] Detector: HA724228, Human Interferon alpha 2 Rabbit mAb [PSH21-61] The concentrations of IFN alpha-2 were measured in duplicates, interpolated from the IFN alpha-2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.