SP-D / SFTPD Recombinant Rabbit Monoclonal Antibody [PSH21-62]
cat.: HA724229
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr, IP, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH21-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse SFTPD aa 1-374. |
| Positive control: | Mouse lung tissue lysate, Rat lung tissue lysate, mouse lung tissue, rat lung tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, surface film. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IP IF-Tissue |
1:5,000 1:1,000 1:200 1-2μg/sample 1:200 |
| Uniprot #: | SwissProt: P50404 Mouse | P35248 Rat |
| Alternative names: | COLEC 7 COLEC7 Collectin-7 Collectin7 Lung surfactant protein D PSP D PSP-D PSP-D Surfactant protein D PSPD Pulmonary surfactant apoprotein Pulmonary surfactant associated protein D Pulmonary surfactant associated protein PSP-D Pulmonary surfactant-associated protein D SFTP 4 SFTP4 SFTPD SFTPD_HUMAN SP D SP-D Surfactant associated protein pulmonary 4 Surfactant protein D Surfactant pulmonary associated protein D |
Images
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Fig1:
Western blot analysis of SP-D / SFTPD on different lysates with Rabbit anti-SP-D / SFTPD antibody (HA724229) at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate (40 µg/Lane) Lane 2: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane) Lane 3: Rat lung tissue lysate (40 µg/Lane) Lane 4: Rat skeletal muscle tissue lysate (negative) (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724229) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: lung Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Rat Site: lung Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SP-D / SFTPD antibody (HA724229) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724229) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue (negative) with Rabbit anti-SP-D / SFTPD antibody (HA724229) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724229) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-SP-D / SFTPD antibody (HA724229) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724229) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue (negative) with Rabbit anti-SP-D / SFTPD antibody (HA724229) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724229) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
SP-D / SFTPD was immunoprecipitated from 0.2 mg rat lung tissue lysate with HA724229 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724229 at 1/10,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Rat lung tissue lysate (input) Lane 2: HA724229 IP in rat lung tissue lysate Lane 3: Rabbit IgG instead of HA724229 in rat lung tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 46 seconds; ECL: K1801 |
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Fig9:
Application: IF-Tissue Species: Mouse Site: lung Sample: Paraffin-embedded section Antibody concentration: 1/200 |
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Fig10:
Application: IF-Tissue Species: Rat Site: lung Sample: Paraffin-embedded section Antibody concentration: 1/200 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.