PCSK9 Recombinant Rabbit Monoclonal Antibody [PSH21-65]
cat.: HA724232
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH21-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse PCSK9 aa 1-694. |
| Positive control: | Huh7 cell lysate, HeLa cell lysate, BxPC-3 cell lysate, A431 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Mouse colon tissue lysate, Huh7. |
| Subcellular location: | Cytoplasm, Secreted, Endosome, Lysosome, Cell surface, Endoplasmic reticulum, Golgi apparatus. |
| Recommended Dilutions:
WB FC IP |
1:5,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q8NBP7 Human | Q80W65 Mouse |
| Alternative names: | Convertase subtilisin/kexin type 9 preproprotein FH3 HCHOLA3 Hypercholesterolemia autosomal dominant 3 LDLCQ1 NARC 1 NARC-1 NARC1 Neural apoptosis regulated convertase 1 Neural apoptosis-regulated convertase 1 PC 9 PC9 PCSK 9 PCSK9 PCSK9_HUMAN Proprotein convertase 9 Proprotein convertase PC9 Proprotein convertase subtilisin/kexin type 9 PSEC0052 Subtilisin/kexin like protease PC9 Subtilisin/kexin-like protease PC9 |
Images
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Fig1:
Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA724232) at 1/5,000 dilution. Lane 1: Huh7 cell lysate Lane 2: SH-SY5Y cell lysate (negative) Lane 3: HeLa cell lysate Lane 4: BxPC-3 cell lysate Lane 6: A431 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 74 kDa Observed band size: 62/74 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724232) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA724232) at 1/5,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse colon tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 74 kDa Observed band size: 62/74 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724232) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Flow cytometric analysis of SH-SY5Y (left, negative) and Huh7 (right, positive) cells labeling PCSK9. Cells were fixed and permeabilized. Then stained with the primary antibody (HA724232, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig4:
PCSK9 was immunoprecipitated from 0.2 mg Huh7 cell lysate with HA724232 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724232 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Huh7 cell lysate (input) Lane 2: HA724232 IP in Huh7 cell lysate Lane 3: Rabbit IgG instead of HA724232 in Huh7 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 19 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.