Mouse Albumin Recombinant Rabbit Monoclonal Antibody [PSH21-67]
cat.: HA724238
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Albumin aa 25-608. |
| Positive control: | Recombinant Mouse Albumin protein. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-68] to Mouse Albumin antibody (Detector) (HA724239) and Recombinant Mouse Albumin protein as the standard. The reference range value is 31.3-8,000 pg/mL. |
| Uniprot #: | SwissProt: P07724 Mouse |
| Alternative names: | alb Albumin (32 AA) Albumin (AA 34) cell growth inhibiting protein 42 DKFZp779N1935 GIG20 GIG42 growth-inhibiting protein 20 OTTHUMP00000220436 OTTHUMP00000220438 OTTHUMP00000220439 PRO0883 PRO0903 PRO1341 PRO1708 PRO2044 PRO2619 PRO2675 Serum albumin UNQ696/PRO1341 |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse Albumin matched pair antibodies Capture: HA724238, Mouse Albumin Rabbit mAb [PSH21-67] Detector: HA724239, Mouse Albumin Rabbit mAb [PSH21-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724238) diluted in carbonate/bicarbonate buffer, at a concentMouseion of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Albumin protein starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA724239, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstMousee for 10 minutes at room tempeMouseure in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Albumin in mouse serum samples and Cculture supernatant. Capture: HA724238, Mouse Albumin Rabbit mAb [PSH21-67] Detector: HA724239, Mouse Albumin Rabbit mAb [PSH21-68] The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.00001% and L-929 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 44.7 mg/ml in mouse serum and undetectable in L-929 cell culture supernatant. |
|
Fig3:
Interpolated concentrations of spiked Albumin in mouse cell culture media samples. Capture: HA724238, Mouse Albumin Rabbit mAb [PSH21-67] Detector: HA724239, Mouse Albumin Rabbit mAb [PSH21-68] The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.