Human/Monkey Coagulation factor VIII Recombinant Rabbit Monoclonal Antibody [PSH21-70]
cat.: HA724241
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Coagulation factor VIII aa 760-1332 (HA211190). |
| Positive control: | Recombinant Human Coagulation factor VIII protein (HA211190). |
| Subcellular location: | Secreted, extracellular space. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-69] to Human/Monkey Coagulation factor VIII antibody (Capture) (HA724240) and Recombinant Human Coagulation factor VIII protein (HA211190) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: P00451 Human |
| Alternative names: | AHF Antihemophilic factor Coagulation factor VIII coagulation factor VIII procoagulant component coagulation factor VIIIc DXS1253E F8 F8b F8c FA8_HUMAN factor VIII F8B Factor VIIIa light chain FactorVIII FVIII Hema Hemophilia A Hemophilia classic OTTHUMP00000061446 OTTHUMP00000196174 Procoagulant component |
Images
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Fig1:
Sandwich ELISA analysis of Human Coagulation factor VIII matched pair antibodies Capture: HA724240, Human/Monkey Coagulation factor VIII Rabbit mAb [PSH21-69] Detector: HA724241, Human/Monkey Coagulation factor VIII Rabbit mAb [PSH21-70] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724240) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Coagulation factor VIII protein (HA211190) protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA724241, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Coagulation factor VIII in Human serum. Capture: HA724240, Human/Monkey Coagulation factor VIII Rabbit mAb [PSH21-69] Detector: HA724241, Human/Monkey Coagulation factor VIII Rabbit mAb [PSH21-70] Interpolated concentration of native Coagulation factor VIII was measured in duplicate at different sample concentrations and interpolated from the Coagulation factor VIII standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Coagulation factor VIII concentration was determined to be 31,715 pg/ml in Human serum. |
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Fig3:
Interpolated concentrations of native Coagulation factor VIII in monkey serum. Capture: HA724240, Human/Monkey Coagulation factor VIII Rabbit mAb [PSH21-69] Detector: HA724241, Human/Monkey Coagulation factor VIII Rabbit mAb [PSH21-70] Interpolated concentration of native Coagulation factor VIII was measured in duplicate at different sample concentrations and interpolated from the Coagulation factor VIII standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Coagulation factor VIII concentration was determined to be 2,697 pg/ml in monkey serum. |
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Fig4:
Interpolated concentrations of spiked Coagulation factor VIII in cell culture media samples. Capture: HA724240, Human/Monkey Coagulation factor VIII Rabbit mAb [PSH21-69] Detector: HA724241, Human/Monkey Coagulation factor VIII Rabbit mAb [PSH21-70] The concentrations of Coagulation factor VIII were measured in duplicates, interpolated from the Coagulation factor VIII standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.