UMOD Recombinant Rabbit Monoclonal Antibody [PSH21-74]
cat.: HA724245
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH21-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human UMOD aa 1-640. |
| Positive control: | Mouse urine, rat urine, human urine, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Apical cell membrane, Basolateral cell membrane, Cell projection, cilium membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:5,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P07911 Human | Q91X17 Mouse | P27590 Rat |
| Alternative names: | ADMCKD2 FJHN HNFJ HNFJ1 MCKD2 medullary cystic kidney disease 2 (autosomal dominant) Tamm Horsfall glycoprotein Tamm Horsfall urinary glycoprotein Tamm-Horsfall urinary glycoprotein THGP THP Umod Urehd1 urehr4 UROM_HUMAN uromodulin (uromucoid, Tamm-Horsfall glycoprotein) Uromodulin Uromodulin, secreted form Uromucoid |
Images
|
Fig1:
Western blot analysis of UMOD on different lysates with Rabbit anti-UMOD antibody (HA724245) at 1/5,000 dilution. Lane 1: Mouse urine Lane 2: Rat urine Lysates/proteins at 30 µg/Lane. Predicted band size: 70 kDa Observed band size: 110 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724245) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of UMOD on human urine with Rabbit anti-UMOD antibody (HA724245) at 1/5,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 70 kDa Observed band size: 110 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724245) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-UMOD antibody (HA724245) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724245) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-UMOD antibody (HA724245) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724245) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Application: IF-Tissue Species: Mouse Site: kidney Sample: Paraffin-embedded section Antibody concentration: 1/200 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.