CD25 / IL-2 Receptor alpha Recombinant Rabbit Monoclonal Antibody [PSH21-78]
cat.: HA724249
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | FC |
| Clonality: | Monoclonal |
| Clone number: | PSH21-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse IL-2 Receptor alpha aa 1-236. |
| Positive control: | Mouse splenocytes, CTLL-2. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
FC |
1:1,000 |
| Uniprot #: | SwissProt: P01590 Mouse |
| Alternative names: | Interleukin 2 receptor alpha chain CD25 CD25 antigen IDDM10 IL 2 receptor alpha subunit IL-2 receptor subunit alpha IL-2-RA IL-2R subunit alpha IL2 RA IL2 Receptor alpha IL2-RA IL2R IL2R, alpha chain IL2RA IMD41 Interleukin 2 receptor alpha Interleukin 2 receptor Interleukin-2 receptor subunit alpha p55 t-cell growth factor receptor TAC TAC antigen TCGFR |
Images
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Fig1:
Flow cytometric analysis of mouse splenocytes labeling CD25 / IL-2 Receptor alpha (HA724249) and CD4-FITC. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA724249, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 647 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1123) at 1/1,000 dilution for 30 minutes at +4℃. Compared with Rabbit IgG Isotype Control (iFluor™ 647, 1/1,000). |
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Fig2:
Flow cytometric analysis of C2C12 (left, negative) and CTLL-2 (right, positive) cells labeling CD25 / IL-2 Receptor alpha. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA724249, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.