Mouse/Rat CD38 Recombinant Rabbit Monoclonal Antibody [PSH21-81]
cat.: HA724252
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: ELISA(Det)
Clonality: Monoclonal
Clone number: PSH21-81
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Mouse CD38 aa 45-304 (HA211383).
Positive control: Recombinant Mouse CD38 protein (HA211383).
Subcellular location: Membrane.
Recommended Dilutions:
  ELISA(Det)
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-80] to Mouse CD38 antibody (Capture) (HA724251) or Rabbit monoclonal [PSH21-82] to Mouse/Rat CD38 antibody (Capture) (HA724253) and Recombinant Mouse CD38 protein (HA211383) as the standard. The reference range value is 5.9-1,500 pg/mL.
Uniprot #: SwissProt: P56528 Mouse | Q64244 Rat
Alternative names: Acute lymphoblastic leukemia cells antigen CD38 ADP ribosyl cyclase 1 ADP ribosyl cyclase ADP ribosyl cyclase/cyclic ADP-ribose hydrolase ADP-ribosyl cyclase 1 ADPRC 1 ADPRC1 cADPr hydrolase 1 CD 38 CD38 CD38 antigen (p45) CD38 antigen CD38 molecule Cd38-rs1 CD38H Cyclic ADP ribose hydrolase Cyclic ADP ribose hydrolase 1 Cyclic ADP-ribose hydrolase 1 EC 3.2.2.5 Ecto nicotinamide adenine dinucleotide glycohydrolase I-19 I19 (mouse) Lymphocyte differentiation antigen CD38 NAD(+) nucleosidase NIM-R5 antigen NIMR5 antigen (mouse) OTTHUMP00000158633 OTTHUMP00000217743 p45 T10
Images
HA724252_1.png Fig1: Sandwich ELISA analysis of Mouse CD38 matched pair antibodies

Capture: HA724251, Mouse CD38 Rabbit mAb [PSH21-80]
Detector: HA724252, Mouse/Rat CD38 Rabbit mAb [PSH21-81]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724251) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD38 protein (HA211383) starting from 1,500 pg/ml to 0 pg/ml and detect antibody (HA724252, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724252_2.png Fig2: Sandwich ELISA analysis of Mouse CD38 matched pair antibodies

Capture: HA724253, Mouse/Rat CD38 Rabbit mAb [PSH21-82]
Detector: HA724252, Mouse/Rat CD38 Rabbit mAb [PSH21-81]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724253) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD38 protein (HA211383) starting from 1,500 pg/ml to 0 pg/ml and detect antibody (HA724252, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724252_3.png Fig3: Interpolated concentrations of native CD38 in Mouse spleen and mouse large intestine tissue extract samples based on a 1000 µg/ml extract load.

Capture: HA724251, Mouse CD38 Rabbit mAb [PSH21-80]
Detector: HA724252, Mouse/Rat CD38 Rabbit mAb [PSH21-81]

Interpolated concentration of native CD38 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 27,873 pg/ml in mouse spleen tissue extract and 19,294 pg/ml in mouse large intestine tissue extract.
HA724252_4.png Fig4: Interpolated concentrations of native CD38 in Mouse spleen and mouse large intestine tissue extract samples based on a 1000 µg/ml extract load.

Capture: HA724253, Mouse/Rat CD38 Rabbit mAb [PSH21-82]
Detector: HA724252, Mouse/Rat CD38 Rabbit mAb [PSH21-81]

Interpolated concentration of native CD38 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 46,466 pg/ml in mouse spleen tissue extract and 17,610 pg/ml in mouse large intestine tissue extract.
HA724252_5.png Fig5: Interpolated concentrations of native CD38 in Rat spleen tissue extract samples based on a 1000 µg/ml extract load.

Capture: HA724253, Mouse/Rat CD38 Rabbit mAb [PSH21-82]
Detector: HA724252, Mouse/Rat CD38 Rabbit mAb [PSH21-81]

Interpolated concentration of native CD38 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 2,727 pg/ml in rat spleen tissue extract.
HA724252_6.png Fig6: Interpolated concentrations of spiked CD38 in cell culture media samples.

Capture: HA724251, Mouse CD38 Rabbit mAb [PSH21-80]
Detector: HA724252, Mouse/Rat CD38 Rabbit mAb [PSH21-81]

The concentrations of CD38 were measured in duplicates, interpolated from the CD38 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA724252_7.png Fig7: Interpolated concentrations of spiked CD38 in cell culture media samples.

Capture: HA724253, Mouse/Rat CD38 Rabbit mAb [PSH21-82]
Detector: HA724252, Mouse/Rat CD38 Rabbit mAb [PSH21-81]

The concentrations of CD38 were measured in duplicates, interpolated from the CD38 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.