Mouse/Rat VEGFA Recombinant Rabbit Monoclonal Antibody [PSH21-89]
cat.: HA724258
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat VEGFA aa 27-190 (HA211331). |
| Positive control: | Recombinant Rat VEGFA protein (HA211331). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-87] to Mouse/Rat VEGFA antibody (Capture) (HA724256) and Recombinant Rat VEGFA protein (HA211331) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: Q00731 Mouse | P16612 Rat |
| Alternative names: | Vascular endothelial growth factor A VEGF-A Vascular permeability factor VPF Vegfa Vegf |
Images
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Fig1:
Sandwich ELISA analysis of Rat VEGFA matched pair antibodies Capture: HA724256, Mouse/Rat VEGFA Rabbit mAb [PSH21-87] Detector: HA724258, Mouse/Rat VEGFA Rabbit mAb [PSH21-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724256) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat VEGFA protein (HA211331) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA724258, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native VEGFA in Mouse lung and Rat lung supernatant. Capture: HA724256, Mouse/Rat VEGFA Rabbit mAb [PSH21-87] Detector: HA724258, Mouse/Rat VEGFA Rabbit mAb [PSH21-89] Interpolated concentration of native VEGFA was measured in duplicate at different sample concentrations and interpolated from the VEGFA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean VEGFA concentration was determined to be 950 pg/ml in Mouse lung supernatant and 4,045 pg/ml in Rat lung supernatant. |
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Fig3:
Interpolated concentrations of spiked VEGFA in cell culture media samples. Capture: HA724256, Mouse/Rat VEGFA Rabbit mAb [PSH21-87] Detector: HA724258, Mouse/Rat VEGFA Rabbit mAb [PSH21-89] The concentrations of VEGFA were measured in duplicates, interpolated from the VEGFA standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.