Mouse CD200 / OX2 Recombinant Rabbit Monoclonal Antibody [PSH21-90]
cat.: HA724259
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CD200 / OX2 aa 31-232 (HA211380). |
| Positive control: | Recombinant Mouse CD200 / OX2 protein (HA211380). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-91] to Mouse CD200 / OX2 antibody (Detector) (HA724260) and Recombinant Mouse CD200 / OX2 protein (HA211380) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: O54901 Mouse |
| Alternative names: | Antigen identified by monoclonal MRC OX 2 CD200 CD200 antigen CD200 molecule MOX1 MOX2 MRC MRC OX 2 antigen My033 OX 2 OX 2 membrane glycoprotein precursor OX-2 membrane glycoprotein OX2G |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse CD200 / OX2 matched pair antibodies Capture: HA724259, Mouse CD200 / OX2 Rabbit mAb [PSH21-90] Detector: HA724260, Mouse CD200 / OX2 Rabbit mAb [PSH21-91] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724259) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD200 / OX2 protein (HA211380) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA724260, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CD200 in mouse samples. Capture: HA724259, Mouse CD200 / OX2 Rabbit mAb [PSH21-90] Detector: HA724260, Mouse CD200 / OX2 Rabbit mAb [PSH21-91] Interpolated concentration of native CD200 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean concentration was determined to be 5,866 pg/mL in mouse serum and 809 pg/ml in C2C12 cell culture supernatant. There was no detectable signal in Neuro-2a cell culture supernatant. |
|
Fig3:
Interpolated concentrations of spiked CD200 in cell culture media samples. Capture: HA724259, Mouse CD200 / OX2 Rabbit mAb [PSH21-90] Detector: HA724260, Mouse CD200 / OX2 Rabbit mAb [PSH21-91] The concentrations of CD200 were measured in duplicates, interpolated from the CD200 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.