Biotin Conjugated Mouse/Rat P-Cadherin Recombinant Rabbit Monoclonal Antibody [PSH21-93]
cat.: HA724262B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH21-93 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse P-Cadherin aa 100-647 (HA211188). |
| Positive control: | Recombinant Mouse P-Cadherin protein (HA211188). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-92] to Mouse/Rat P-Cadherin antibody (Capture) (HA724261) and Recombinant Mouse P-Cadherin protein (HA211188) as the standard. The reference range value is 78.1-20,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P10287 Mouse Entrez Gene: 116777 Rat |
| Alternative names: | Cadherin-3 Placental cadherin P-cadherin Cdh3 Cdhp |
Images
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Fig1:
Sandwich ELISA analysis of Mouse P-Cadherin matched pair antibodies Capture: HA724261, Mouse/Rat P-Cadherin Rabbit mAb [PSH21-92] Detector: HA724262, Mouse/Rat P-Cadherin Rabbit mAb [PSH21-93] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724261) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse P-Cadherin protein (HA211188) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA724262, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native P-Cadherin in Mouse and Rat serum. Capture: HA724261, Mouse/Rat P-Cadherin Rabbit mAb [PSH21-92] Detector: HA724262, Mouse/Rat P-Cadherin Rabbit mAb [PSH21-93] Interpolated concentration of native P-Cadherin was measured in duplicate at different sample concentrations and interpolated from the P-Cadherin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean P-Cadherin concentration was determined to be 110,094 pg/ml in Mouse serum and 39,985 pg/ml in Rat serum. |
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Fig3:
Interpolated concentrations of spiked P-Cadherin in cell culture media samples. Capture: HA724261, Mouse/Rat P-Cadherin Rabbit mAb [PSH21-92] Detector: HA724262, Mouse/Rat P-Cadherin Rabbit mAb [PSH21-93] The concentrations of P-Cadherin were measured in duplicates, interpolated from the P-Cadherin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.