Biotin Conjugated Mouse CD80 Recombinant Rabbit Monoclonal Antibody [PSH21-95]
cat.: HA724264B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH21-95 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CD80 aa 38-246 (HA211384). |
| Positive control: | Recombinant Mouse CD80 protein (HA211384). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-94] to Mouse CD80 antibody (Capture) (HA724263) and Recombinant Mouse CD80 protein (HA211384) as the standard. The reference range value is 11.7-1,500 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q00609 Mouse |
| Alternative names: | Activation B7-1 antigen B lymphocyte activation antigen B7 B7 B7-1 B7-1 antigen B7.1 BB1 CD28 antigen ligand 1 CD28LG CD28LG1 CD80 CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) CD80 antigen CD80 molecule Costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 LAB7 T-lymphocyte activation antigen CD80 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CD80 matched pair antibodies Capture: HA724263, Mouse CD80 Rabbit mAb [PSH21-94] Detector: HA724264, Mouse CD80 Rabbit mAb [PSH21-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724263) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD80 protein (HA211384) starting from 1500 pg/ml to 0 pg/ml and detect antibody (HA724264, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD80 in mouse samples. Capture: HA724263, Mouse CD80 Rabbit mAb [PSH21-94] Detector: HA724264, Mouse CD80 Rabbit mAb [PSH21-95] Interpolated concentration of native CD80 was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 2,139 pg/mL in mouse serum and 132 pg/ml in C2C12 cell supernatant. There was no detectable signal in Neuro-2a cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked CD80 in cell culture media samples. Capture: HA724263, Mouse CD80 Rabbit mAb [PSH21-94] Detector: HA724264, Mouse CD80 Rabbit mAb [PSH21-95] The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.