TATA binding protein TBP Recombinant Rabbit Monoclonal Antibody [PSH22-13]
cat.: HA724274
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Green monkey
Applications: WB, IF-Cell, FC, IP
Clonality: Monoclonal
Clone number: PSH22-13
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Positive control: HeLa cell lysate, 293T cell lysate, HCT 116 cell lysate, COS-1 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse testis tissue lysate, Mouse colon tissue lysate, Rat testis tissue lysate, HeLa, NIH/3T3, C6.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  FC
  IP
1:5,000
1:250
1:1,000
1-2μg/sample
Uniprot #: SwissProt: P20226 Human | P29037 Mouse
Entrez Gene: 117526 Rat
Alternative names: GTF2D GTF2D1 HDL4 MGC117320 MGC126054 MGC126055 SCA17 TATA binding factor TATA box factor TATA sequence binding protein TATA sequence-binding protein TATA-binding factor TATA-box binding protein N-terminal domain TATA-box factor TATA-box-binding protein TBP TBP_HUMAN TFIID Transcription initiation factor TFIID TBP subunit
Images
HA724274_1.jpg Fig1: Western blot analysis of TATA binding protein TBP on different lysates with Rabbit anti-TATA binding protein TBP antibody (HA724274) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: 293T cell lysate (15 µg/Lane)
Lane 3: HCT 116 cell lysate (15 µg/Lane)
Lane 4: COS-1 cell lysate (15 µg/Lane)
Lane 5: C2C12 cell lysate (15 µg/Lane)
Lane 6: NIH/3T3 cell lysate (15 µg/Lane)
Lane 7: C6 cell lysate (15 µg/Lane)

Predicted band size: 38 kDa
Observed band size: 35-40 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724274) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA724274_2.jpg Fig2: Western blot analysis of TATA binding protein TBP on different lysates with Rabbit anti-TATA binding protein TBP antibody (HA724274) at 1/5,000 dilution.

Lane 1: Mouse testis tissue lysate
Lane 2: Mouse colon tissue lysate
Lane 3: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724274) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA724274_3.jpg Fig3: Immunocytochemistry analysis of HeLa cells labeling TATA binding protein TBP with Rabbit anti-TATA binding protein TBP antibody (HA724274) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TATA binding protein TBP antibody (HA724274) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA724274_4.jpg Fig4: Immunocytochemistry analysis of NIH/3T3 cells labeling TATA binding protein TBP with Rabbit anti-TATA binding protein TBP antibody (HA724274) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TATA binding protein TBP antibody (HA724274) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA724274_5.jpg Fig5: Immunocytochemistry analysis of C6 cells labeling TATA binding protein TBP with Rabbit anti-TATA binding protein TBP antibody (HA724274) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TATA binding protein TBP antibody (HA724274) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA724274_6.jpg Fig6: Flow cytometric analysis of HeLa cells labeling TATA binding protein TBP.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA724274, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA724274_7.jpg Fig7: Flow cytometric analysis of NIH/3T3 cells labeling TATA binding protein TBP.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA724274, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA724274_8.jpg Fig8: Flow cytometric analysis of C6 cells labeling TATA binding protein TBP.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA724274, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA724274_9.jpg Fig9: TATA binding protein TBP was immunoprecipitated from 0.2 mg HeLa cell lysate with HA724274 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724274 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: HA724274 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA724274 in HeLa cell lysate

Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 20 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.