HA724275

TROP2 Recombinant Rabbit Monoclonal Antibody [PSH22-14]

cat.: HA724275

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-Fr, IHC-P, IF-Tissue
Clonality:	Monoclonal
Clone number:	PSH22-14

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration:	0.5ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 36 kDa
Isotype:	IgG
Positive control:	MDA-MB-468 cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, A431 cell lysate, Mouse skin tissue lysate, Mouse lung tissue lysate, Rat skin tissue lysate, Rat lung tissue lysate, MCF7, human breast cancer tissue, human breast ductal carcinoma tissue, human cervical squamous cell carcinoma tissue, human urothelial carcinoma tissue, human prostate cancer tissue, human kidney tissue, human skin tissue, mouse kidney tissue, mouse skin tissue, rat skin tissue.
Subcellular location:	Membrane.
Recommended Dilutions: WB IF-Cell IHC-Fr IHC-P IF-Tissue	1:2,000-1:5,000 1:2,500 1:500 1:1,000 1:400
Uniprot #:	SwissProt: P09758 Human \| Q8BGV3 Mouse Entrez Gene: 494343 Rat
Alternative names:	Cell surface glycoprotein Trop 2 Cell surface glycoprotein Trop-2 Cell surface glycoprotein Trop2 Epithelial glycoprotein 1 GA733 1 GA7331 M1S 1 M1S1 Membrane component chromosome 1 surface marker 1 Pancreatic carcinoma marker protein GA733 1 Pancreatic carcinoma marker protein GA733-1 Pancreatic carcinoma marker protein GA7331 TACD 2 TACD2_HUMAN TACSTD 2 Tacstd2 Trop 2 Trop2 Tumor associated calcium signal transducer 2 precursor Tumor-associated calcium signal transducer 2

Images

Fig1: Western blot analysis of TROP2 on different lysates with Rabbit anti-TROP2 antibody (HA724275) at 1/5,000 dilution.

Lane 1: MDA-MB-468 cell lysate (10 µg/Lane)
Lane 2: MCF7 cell lysate (10 µg/Lane)
Lane 3: SK-Br-3 cell lysate (10 µg/Lane)
Lane 4: A431 cell lysate (10 µg/Lane)
Lane 5: HEK-293 cell lysate (negative) (10 µg/Lane)

Predicted band size: 36 kDa
Observed band size: 40-60 kDa

Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724275) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Negative control: HEK-293 (PMID: 38250577).

Fig2: Western blot analysis of TROP2 on different lysates with Rabbit anti-TROP2 antibody (HA724275) at 1/5,000 dilution.

Lane 1: Mouse skin tissue lysate (20 µg/Lane)
Lane 2: Mouse lung tissue lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (negative) (20 µg/Lane)
Lane 4: Rat skin tissue lysate (20 µg/Lane)
Lane 5: Rat lung tissue lysate (20 µg/Lane)
Lane 6: Rat liver tissue lysate (negative) (20 µg/Lane)

Predicted band size: 36 kDa
Observed band size: 40-60 kDa

Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724275) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Negative control: liver tissue (PMID: 38250577).

	Fig3: Immunocytochemistry analysis of MCF7 (positive) and HEK-293 (negative) labeling TROP2 with Rabbit anti-TROP2 antibody (HA724275) at 1/2,500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TROP2 antibody (HA724275) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. Negative control: HEK-293 (PMID: 38250577).
	Fig4: Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
	Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig9: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig10: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig11: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig12: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig13: Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig14: Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-TROP2 antibody (HA724275) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA724275) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig15: Application: IF-Tissue Species: Human Site: skin Sample: Paraffin-embedded section Antibody concentration: 1/400
	Fig16: Application: IF-Tissue Species: Rat Site: skin Sample: Paraffin-embedded section Antibody concentration: 1/400

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.