Human IGFBP6 Recombinant Rabbit Monoclonal Antibody [PSH22-19]
cat.: HA724280
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IGFBP6 aa 28-240 (HA211086). |
| Positive control: | Recombinant Human IGFBP6 protein (HA211086). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-18] to Human IGFBP6 antibody (Capture) (HA724279) and Recombinant Human IGFBP6 protein (HA211086) as the standard. The reference range value is 62.5-8,000 pg/mL. |
| Uniprot #: | SwissProt: P24592 Human |
| Alternative names: | IBP 6 IBP-6 IBP6 IBP6_HUMAN IGF binding protein 6 IGF-binding protein 6 IGFBP 6 IGFBP-6 IGFBP6 Insulin like growth factor binding protein 6 Insulin-like growth factor-binding protein 6 |
Images
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Fig1:
Sandwich ELISA analysis of Human IGFBP6 matched pair antibodies Capture: HA724279, Human IGFBP6 Rabbit mAb [PSH22-18] Detector: HA724280, Human IGFBP6 Rabbit mAb [PSH22-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724279) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IGFBP6 protein (HA211086) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA724280, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IGFBP6 in human samples. Capture: HA724279, Human IGFBP6 Rabbit mAb [PSH22-18] Detector: HA724280, Human IGFBP6 Rabbit mAb [PSH22-19] Interpolated concentration of native IGFBP6 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IGFBP6 concentration was determined to be 462,281 pg/mL in human serum and 22,992 pg/ml in HaCaT cell supernatant. There was no detectable signal in THP-1 cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked IGFBP6 in cell culture media samples. Capture: HA724279, Human IGFBP6 Rabbit mAb [PSH22-18] Detector: HA724280, Human IGFBP6 Rabbit mAb [PSH22-19] The concentrations of IGFBP6 were measured in duplicates, interpolated from the IGFBP6 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.