Human Complement factor H Recombinant Rabbit Monoclonal Antibody [PSH22-20]
cat.: HA724281
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-20 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Complement factor H aa 866-1231 (HA211200). |
| Positive control: | Recombinant Human Complement factor H protein (HA211200). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-21] to Human Complement factor H antibody (Detector) (HA724282) and Recombinant Human Complement factor H protein (HA211200) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: P08603 Human |
| Alternative names: | adrenomedullin binding protein age related maculopathy susceptibility 1 AHUS 1 AHUS1 AMBP 1 AMBP1 ARMD 4 ARMD4 ARMS 1 ARMS1 beta 1 H globulin beta 1H beta1H CFAH_HUMAN CFH CFHL 3 CFHL3 Complement factor H complement factor H, isoform b Factor H factor H like 1 FH FHL 1 FHL1 H factor 1 (complement) H factor 1 H factor 2 (complement) HF 1 HF 2 HF HF1 HF2 HUS MGC88246 |
Images
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Fig1:
Sandwich ELISA analysis of Human Complement factor H matched pair antibodies Capture: HA724281, Human Complement factor H Rabbit mAb [PSH22-20] Detector: HA724282, Human Complement factor H Rabbit mAb [PSH22-21] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724281) diluted in carbonate/bicarbonate buffer, at a concentHumanion of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Complement factor H protein (HA211200) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA724282, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstHumane for 10 minutes at room tempeHumanure in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Complement factor H in human serum samples. Capture: HA724281, Human Complement factor H Rabbit mAb [PSH22-20] Detector: HA724282, Human Complement factor H Rabbit mAb [PSH22-21] The concentrations of Complement factor H were measured in duplicates, interpolated from the Complement factor H standard curve and corrected for sample dilution. Undiluted samples are human serum 0.005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement factor H concentration was determined to be 124.6 µg/ml in human serum. |
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Fig3:
Interpolated concentrations of spiked Complement factor H in human cell culture media samples. Capture: HA724281, Human Complement factor H Rabbit mAb [PSH22-20] Detector: HA724282, Human Complement factor H Rabbit mAb [PSH22-21] The concentrations of Complement factor H were measured in duplicates, interpolated from the Complement factor H standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media without FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.