Human TLR3 Recombinant Rabbit Monoclonal Antibody [PSH22-25]
cat.: HA724286
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TLR3 aa 27-700 (HA211381). |
| Positive control: | Recombinant Human TLR3 protein (HA211381). |
| Subcellular location: | Endoplasmic reticulum membrane, Endosome membrane, Early endosome. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-24] to Human TLR3 antibody (Capture) (HA724285) and Recombinant Human TLR3 protein (HA211381) as the standard. The reference range value is 39-10,000 pg/mL. |
| Uniprot #: | SwissProt: O15455 Human |
| Alternative names: | CD283 CD283 antigen IIAE2 TLR 3 Tlr3 TLR3_HUMAN Toll Like Receptor 3 Toll-like receptor 3 |
Images
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Fig1:
Sandwich ELISA analysis of Human TLR3 matched pair antibodies Capture: HA724285, Human TLR3 Rabbit mAb [PSH22-24] Detector: HA724286, Human TLR3 Rabbit mAb [PSH22-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724285) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TLR3 protein (HA211381) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA724286, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native TLR3 in A431, HeLa and A549 cell extract samples based on a 1000 µg/ml extract load. Capture: HA724285, Human TLR3 Rabbit mAb [PSH22-24] Detector: HA724286, Human TLR3 Rabbit mAb [PSH22-25] Interpolated concentration of native TLR3 was measured in duplicate at different sample concentrations and interpolated from the TLR3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TLR3 concentration was determined to be 29,603 pg/mL in A431 cell extract, 1,422 pg/mL in HeLa cell extract and 738 pg/mL in A549 cell extract. |
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Fig3:
Interpolated concentrations of spiked TLR3 in cell culture media samples. Capture: HA724285, Human TLR3 Rabbit mAb [PSH22-24] Detector: HA724286, Human TLR3 Rabbit mAb [PSH22-25] The concentrations of TLR3 were measured in duplicates, interpolated from the TLR3 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.