BARD1 Recombinant Rabbit Monoclonal Antibody [PSH22-30]
cat.: HA724291
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH22-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BARD1 aa 1-450. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, HeLa treated with 2μM MG-132 for 18 hours cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q99728 Human |
| Alternative names: | BARD-1 Bard1 BARD1_HUMAN BRCA1 associated RING domain 1 BRCA1 associated RING domain gene 1 BRCA1 associated RING domain protein 1 BRCA1-associated RING domain protein 1 |
Images
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Fig1:
Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (HA724291) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: MDA-MB-231 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 87/100 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724291) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of BARD1 on different lysates with Rabbit anti-BARD1 antibody (HA724291) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 2μM MG-132 for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 87/100 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724291) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
BARD1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA724291 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724291 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA724291 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA724291 in HeLa cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.