INSIG1 Recombinant Rabbit Monoclonal Antibody [PSH22-32]
cat.: HA724293
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH22-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse INSIG1 aa 210-259. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O15503 Human | Q8BGI3 Mouse | Q08755 Rat |
| Alternative names: | Insulin-induced gene 1 protein INSIG-1 INSIG1 |
Images
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Fig1:
Western blot analysis of INSIG1 on different lysates with Rabbit anti-INSIG1 antibody (HA724293) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: Jurkat cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Neuro-2a cell lysate Lane 7: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 30 kDa Observed band size: 45 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724293) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of INSIG1 on different lysates with Rabbit anti-INSIG1 antibody (HA724293) at 1/5,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 30 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724293) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.