Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody [PSH22-36]
cat.: HA724294
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH22-36 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 225 kDa |
| Isotype: | IgG |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue. |
| Subcellular location: | Cell membrane, Cell projection, axon; Cytoplasmic vesicle, Cell projection, podosome. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000-1:5,000 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: Q9UQD0 Human | Q9WTU3 Mouse | O88420 Rat |
| Alternative names: | CerIII CIAT EIEE13 hNa6/Scn8a voltage gated sodium channel MED Motor endplate disease NaCh 6 NaCh6 Nav 1.6 Nbna1 peripheral nerve protein type 4 PN 4 PN4 SCN8A SCN8A_HUMAN Sodium channel protein type 8 alpha subunit Sodium channel protein type 8 subunit alpha Sodium channel protein type VIII alpha subunit Sodium channel protein type VIII subunit alpha Sodium channel voltage gated type VIII alpha Sodium channel voltage gated type VIII alpha polypeptide Sodium channel voltage gated type VIII alpha subunit Voltage gated sodium channel subunit alpha Nav1.6 Voltage-gated sodium channel subunit alpha Nav1.6 |
Images
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Fig1:
Western blot analysis of Nav1.6 / SCN8A on different lysates with Rabbit anti-Nav1.6 / SCN8A antibody (HA724294) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse liver tissue lysate (negative) (no heat) Lane 3: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 225 kDa Observed band size: 260 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724294) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Negative control : Liver (PMID : 11532991) |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Nav1.6 / SCN8A antibody (HA724294) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724294) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Nav1.6 / SCN8A antibody (HA724294) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724294) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Negative control : Liver (PMID : 11532991) |
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Fig4:
Nav1.6 / SCN8A was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA724294 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724294 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA724294 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA724294 in mouse brain tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 1 minute 59 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.