P2X7 Recombinant Rabbit Monoclonal Antibody [PSH22-44]
cat.: HA724296
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH22-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse P2X7 aa 354-595. |
| Positive control: | Mouse brain lysate, Mouse lung tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:25,000 |
| Uniprot #: | SwissProt: Q9Z1M0 Mouse |
| Alternative names: | ATP receptor P2rx7 P2X purinoceptor 7 P2X7 P2Z receptor Purinergic receptor purinergic receptor P2X, ligand gated ion channel, 7 |
Images
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Fig1:
Western blot analysis of P2X7 on different lysates with Rabbit anti-P2X7 antibody (HA724296) at 1/25,000 dilution. Lane 1: Mouse brain lysate (no heat) Lane 2: Mouse skeletal muscle lysate (no heat) (negative) Lane 3: Mouse lung tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Predicted band size: 68 kDa Observed band size: 75 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724296) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Low expression or negative control : Mouse skeletal muscle (PMID: 15254086, PMID: 22415881) |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.