Mouse CD163 Recombinant Rabbit Monoclonal Antibody [PSH22-49]
cat.: HA724299
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CD163 aa 39-1045 (HA211297). |
| Positive control: | Recombinant Mouse CD163 protein (HA211297). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-48] to Mouse CD163 antibody (Capture) (HA724299) and Recombinant Mouse CD163 protein (HA211297) as the standard. The reference range value is 39.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: Q2VLH6 Mouse |
| Alternative names: | CD 163 CD163 CD163 antigen CD163 molecule Hemoglobin scavenger receptor M130 M130 antigen precursor Macrophage associated antigen MM130 OTTHUMP00000238617 OTTHUMP00000238618 OTTHUMP00000238619 OTTHUMP00000238620 SCARI1 Scavenger receptor cysteine rich type 1 protein M130 sCD163 Soluble CD163 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CD163 matched pair antibodies Capture: HA724298, Mouse CD163 Rabbit mAb [PSH22-48] Detector: HA724299, Mouse CD163 Rabbit mAb [PSH22-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724298) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD163 protein (HA211297) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724299, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD163 in mouse serum samples and J774A.1 cell culture supernatant. Capture: HA724298, Mouse CD163 Rabbit mAb [PSH22-48] Detector: HA724299, Mouse CD163 Rabbit mAb [PSH22-49] The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 2% and J774A.1 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 364 ng/ml in mouse serum and undetectable in J774A.1 cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked CD163 in mouse cell culture media samples. Capture: HA724298, Mouse CD163 Rabbit mAb [PSH22-48] Detector: HA724299, Mouse CD163 Rabbit mAb [PSH22-49] The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.