BAP1 Recombinant Rabbit Monoclonal Antibody [PSH22-53]
cat.: HA724303
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: PSH22-53
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 80 kDa
Isotype: IgG
Immunogen: Recombinant protein within human BAP1 aa 201-550.
Positive control: HeLa cell lysate, A375 cell lysate, MOLT-4 cell lysate, PC-3 cell lysate, A549 cell lysate, MDA-MB-231 cell lysate, Mouse testis tissue lysate, Mouse brain tissue lysate, Mouse ovary tissue lysate, E17 mouse placenta tissue lysate, Rat testis tissue lysate, Rat brain tissue lysate, Rat ovary tissue lysate.
Subcellular location: Cytoplasm, Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IP
1:5,000
1-2μg/sample
Uniprot #: SwissProt: Q92560 Human | Q99PU7 Mouse | D3ZHS6 Rat
Alternative names: BAP 1 Bap1 BAP1_HUMAN BRCA 1 associated protein 1 BRCA1 associated protein 1 BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) BRCA1-associated protein 1 Cerebral protein 13 Cerebral protein 6 DKFZp686N04275 FLJ35406 FLJ37180 HUCEP 13 Hucep 6 HUCEP13 Hucep6 KIAA0272 TPDS Ubiquitin carboxy terminal hydrolase Ubiquitin carboxyl terminal hydrolase BAP 1 Ubiquitin carboxyl terminal hydrolase BAP1 Ubiquitin carboxyl-terminal hydrolase BAP1 UCHL2
Images
HA724303_1.jpg Fig1: Western blot analysis of BAP1 on different lysates with Rabbit anti-BAP1 antibody (HA724303) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: NCI-H226 cell lysate (negative) (15 µg/Lane)
Lane 3: A375 cell lysate (15 µg/Lane)
Lane 4: MOLT-4 cell lysate (15 µg/Lane)
Lane 5: PC-3 cell lysate (15 µg/Lane)
Lane 6: A549 cell lysate (15 µg/Lane)
Lane 7: MDA-MB-231 cell lysate (15 µg/Lane)

Predicted band size: 80 kDa
Observed band size: 95 kDa

Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724303) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA724303_2.jpg Fig2: Western blot analysis of BAP1 on different lysates with Rabbit anti-BAP1 antibody (HA724303) at 1/5,000 dilution.

Lane 1: Mouse testis tissue lysate (30 µg/Lane)
Lane 2: Mouse brain tissue lysate (30 µg/Lane)
Lane 3: Mouse ovary tissue lysate (30 µg/Lane)
Lane 4: E17 mouse placenta tissue lysate (30 µg/Lane)
Lane 5: Rat testis tissue lysate (30 µg/Lane)
Lane 6: Rat brain tissue lysate (30 µg/Lane)
Lane 7: Rat ovary tissue lysate (30 µg/Lane)

Predicted band size: 80 kDa
Observed band size: 95 kDa

Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724303) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA724303_3.jpg Fig3: BAP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA724303 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724303 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: HA724303 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA724303 in HeLa cell lysate

Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 30 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.