CXCL5 Recombinant Rabbit Monoclonal Antibody [PSH22-58]
cat.: HA724308
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH22-58 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CXCL5 aa 1-114. |
| Positive control: | A549 treated with 10ng/mL TNF-α and 10nM TPA for 24 hours cell lysate, A549 cells starved overnight then treated with 10ng/mL TNF-α and 10nM TPA for 24 hours add 300ng/mL BFA for last 3 hours. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:100 1:10,000 |
| Uniprot #: | SwissProt: P42830 Human |
| Alternative names: | AMCFII C-X-C motif chemokine 5 C-X-C motif chemokine ligand 5 Chemokine (C X C motif) ligand 5 chemokine (C-X-C motif) ligand 5 Cxcl5 CXCL5_HUMAN ENA 78 ENA-78 (8-78) ENA-78(1-78) ENA-78(9-78) ENA78 Epithelial derived neutrophil activating protein 78 Epithelial-derived neutrophil-activating protein 78 Lipopolysaccharide-induced CXC chemokine Neutrophil activating peptide ENA 78 Neutrophil activating protein 78 Neutrophil-activating peptide ENA-78 neutrophil-activating protein 78 SCYB5 Small inducible cytokine B5 small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) small inducible cytokine subfamily B, member 5 Small-inducible cytokine B5 |
Images
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Fig1:
Western blot analysis of CXCL5 on different lysates with Rabbit anti-CXCL5 antibody (HA724308) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with 10ng/mL TNF-α and 10nM TPA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724308) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A549 cells untreated / starved overnight then treated with 10ng/mL TNF-α and 10nM TPA for 24 hours add 300ng/mL BFA for last 3 hours labeling CXCL5 with Rabbit anti-CXCL5 antibody (HA724308) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL5 antibody (HA724308) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of A549 cells untreated (left) / starved overnight then treated with 10ng/mL TNF-α and 10nM TPA for 24 hours add 300ng/mL BFA for last 3 hours (right) labeling CXCL5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA724308, 0.1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.