MTBR-TAU 243 lsoform 2N4R(236-250) Recombinant Rabbit Monoclonal Antibody [PSH22-59]
cat.: HA724309
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Pig, Human |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH22-59 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MAPT aa 236-250. |
| Positive control: | Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, Pig brain tissue lysate, Neuro-2a. |
| Subcellular location: | Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted. |
| Recommended Dilutions:
WB IP FC |
1:5,000 1-2μg/sample 1:1,000 |
| Uniprot #: | SwissProt: P10637 Mouse | P19332 Rat |
| Alternative names: | AI413597 AW045860 DDPAC FLJ31424 FTDP 17 G protein beta1/gamma2 subunit interacting factor 1 MAPT MAPTL MGC134287 MGC138549 MGC156663 Microtubule associated protein tau Microtubule associated protein tau isoform 4 Microtubule-associated protein tau MSTD Mtapt MTBT1 MTBT2 Neurofibrillary tangle protein Paired helical filament tau Paired helical filament-tau PHF tau PHF-tau PPND PPP1R103 Protein phosphatase 1, regulatory subunit 103 pTau RNPTAU TAU Tauopathy and respiratory failure, included |
Images
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Fig1:
Western blot analysis of MTBR-TAU 243 lsoform 2N4R(236-250) on different lysates with Rabbit anti-MTBR-TAU 243 lsoform 2N4R(236-250) antibody (HA724309) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse spleen tissue lysate (negative) Lane 3: Mouse cerebellum tissue lysate Lane 4: Mouse testis tissue lysate (negative) Lane 5: Rat brain tissue lysate Lane 6: Rat spleen tissue lysate (negative) Lane 7: Rat cerebellum tissue lysate Lane 8: Rat testis tissue lysate (negative) Lane 9: Pig brain tissue lysate Lane 10: Pig spleen tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Observed band size: 50-80 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724309) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Negative control : Spleen, Testis (PMID: 24309898, PMID: 24386422) |
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Fig2:
MTBR-TAU 243 lsoform 2N4R(236-250) was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA724309 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724309 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA724309 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA724309 in mouse brain tissue lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 7 seconds; ECL: K1801 |
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Fig3:
Immunoprecipitation (IP) 50 ng of full-length TAU isoform 2N4R protein (1-441 aa) and 200 ng of truncated TAU isoform 2N4R protein (1-256 aa) were incubated with 0.2 mg of negative tissue lysate from mouse spleen, along with HA724309 at 2 µg/10 µl of beads. Western blot was performed from the immunoprecipitate using HA724309 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen (input) Lane 2: HA724309 IP in TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen Lane 3: Rabbit IgG instead of HA724309 in TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen Exposure time: 16 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724309, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Observed band size: 50-80 kDa |
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Fig4:
Flow cytometric analysis of EL4 (left, negative) and Neuro-2a (right, positive) cells labeling MTBR-TAU 243 lsoform 2N4R(236-250). Cells were fixed and permeabilized. Then stained with the primary antibody (HA724309, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5: HA724309 epitope mapping schematic diagram |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.