AGRP Recombinant Rabbit Monoclonal Antibody [PSH22-63]
cat.: HA724311
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH22-63 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AGRP aa 1-132. |
| Positive control: | Mouse hypothalamus (arctic nucleus) tissue, rat hypothalamus (arctic nucleus) tissue. |
| Subcellular location: | Secreted, Golgi apparatus lumen. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:500 1:1,000 |
| Uniprot #: | SwissProt: P56473 Mouse Entrez Gene: 25582 Rat |
| Alternative names: | Agouti-related protein Agrp Agrt Art |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: hypothalamus (arctic nucleus) Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Brain(Paraventricular nucleus) Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724311, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hypothalamus (arctic nucleus) tissue with Rabbit anti-AGRP antibody (HA724311) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724311) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat hypothalamus (arctic nucleus) tissue with Rabbit anti-AGRP antibody (HA724311) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724311) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.