TRPA1 Recombinant Rabbit Monoclonal Antibody [PSH23-56]
cat.: HA724319
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Green monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH23-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRPA1 aa 1-750. |
| Positive control: | U-2 OS (Human osteosarcoma cells) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, SH-SY5Y (Human neuroblastoma cells) cell lysate, IMR-32 (Human neuroblastoma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O75762 Human |
| Alternative names: | ANKTM 1 ANKTM1 Ankyrin-like with transmembrane domains protein 1 Transformation sensitive protein p120 Transformation-sensitive protein p120 Transient receptor potential cation channel subfamily A member 1 TRPA 1 Trpa1 TRPA1_HUMAN |
Images
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Fig1:
Western blot analysis of TRPA1 on different lysates with Rabbit anti-TRPA1 antibody (HA724319) at 1/5,000 dilution. Lane 1: U-2 OS (Human osteosarcoma cells) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate Lane 4: IMR-32 (Human neuroblastoma cells) cell lysate Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724319, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 127 kDa Observed band size: 150 kDa |
|
Fig2:
Western blot analysis of TRPA1 on different lysates with Rabbit anti-TRPA1 antibody (HA724319) at 1/5,000 dilution. Lane 1: SH-SY5Y -si WT cell lysate Lane 2: SH-SY5Y -si TRPA1 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724319, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 127 kDa Observed band size: 150 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.